recombinant fndc5  (Abnova)

Journal: International Journal of Molecular Sciences

Article Title: Cerebral Benefits Induced by Electrical Muscle Stimulation: Evidence from a Human and Rat Study

doi: 10.3390/ijms25031883

Figure Lengend Snippet: Effect of EMS on the FNDC5/irisin pathway. ( A ) Relative protein levels of FNDC5/irisin in quadriceps muscle at 4 h (hatched bar) and 24 h (full bar) in the SHAM (black) and EMS (green) groups. ( B ) Spearman correlation between hippocampal BDNF and quadriceps muscle FNDC5/irisin relative protein expression levels at the 24 h time point for SHAM rats (black circle) and EMS rats (green triangle). ( C , D ) Circulating irisin levels measured by ELISA immediately (tail blood collection, ( C )) and 4 or 24 h (intracardiac blood collection, ( D )) after protocol application. ( E ) Representative immunoblots of pooled immunoprecipitated serum immediately, 4 h, and 24 h following the procedures. ( F ) Schematic signaling pathway activated by irisin upon binding to the integrin receptor. ( G ) Relative protein levels of FNDC5/irisin in the hippocampus 4 and 24 h after the procedures. ( H ) Relative protein levels of p-FAK Tyr397 in the hippocampus at the 4 h and 24 h time points. Corresponding immunoblots are shown on the side of the graphs. NS means no significance.

Article Snippet: FNDC5/irisin , Abcam recombinant anti-FNDC5 antibody rabbit monocolonal [EPR12209] (ab174833) , 1/3000 TBST-Milk (5%).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation, Binding Assay

Journal: International Journal of Molecular Sciences

Article Title: Cerebral Benefits Induced by Electrical Muscle Stimulation: Evidence from a Human and Rat Study

doi: 10.3390/ijms25031883

Figure Lengend Snippet: Primary antibodies.

Article Snippet: FNDC5/irisin , Abcam recombinant anti-FNDC5 antibody rabbit monocolonal [EPR12209] (ab174833) , 1/3000 TBST-Milk (5%).

Techniques: Recombinant, Transduction, Purification

Journal: Scientific Reports

Article Title: Gene Location, Expression, and Function of FNDC5 in Meishan Pigs

doi: 10.1038/s41598-017-08406-y

Figure Lengend Snippet: Identification of DNA sequences encoding intact irisin in porcine genome. ( A ) Amino acid sequence alignment of murine, human, and porcine irisin protein. *: indicating same amino acid; •: indicating missing amino acid. ( B ) DNA sequence encoding the missing 43 amino acids corresponding to the human genome compared to the NCBI database for porcine FNDC5. The missing sequence is divided into two parts, missing part 1 and missing part 2. *: indicating same base; •: indicating missing base. ( C ) Alignment of the missing part 1 DNA sequence encoding the missing 43 amino acids in human genome with DNA sequence of porcine FNDC5 from the NCBI database after adjustment of 10 bases up 5′ end. *: indicating same base; •: indicating missing base.

Article Snippet: Recombinant FNDC5 protein (Cat No: H00252995-P01) was from Abnova.

Techniques: Sequencing

Journal: Scientific Reports

Article Title: Gene Location, Expression, and Function of FNDC5 in Meishan Pigs

doi: 10.1038/s41598-017-08406-y

Figure Lengend Snippet: Detection of five FNDC5 transcripts in Meishan pigs. ( A ) RT-PCR results of irisin sequence in Meishan pigs, NC represents negative control group with no template. ( B ) 5′ RACE nested PCR results of FNDC5 from Meishan pigs, NC represents negative control group with no template. ( C ) 3′ RACE nested PCR results of FNDC5 from Meishan pig, NC represents negative control group with no template. ( D ) RT-PCR results from the full-length FNDC5 in Meishan pigs, NC represents negative control group with no template. ( E ) Different splicing patterns and locations of FNDC5 gene in Meishan pigs. Black bars: exons, black lines: introns. Numbers represent cleavage sites.

Article Snippet: Recombinant FNDC5 protein (Cat No: H00252995-P01) was from Abnova.

Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing, Negative Control, Nested PCR

Journal: Scientific Reports

Article Title: Gene Location, Expression, and Function of FNDC5 in Meishan Pigs

doi: 10.1038/s41598-017-08406-y

Figure Lengend Snippet: Different FNDC5 expression levels in organs and tissues from Meishan pigs. ( A ) Expression levels of FNDC5 in different tissues of Meishan pigs. Data are reported as mean ± SEM (n = 4). ( B ) Western blot results of FNDC5 in different tissues of Meishan pigs. ( C ) Expression levels of FNDC5 in skeletal muscle at different development stages of Meishan pigs. Data are reported as mean ± SEM (n = 4). E: embryo stage; D: days after birth; M: month after birth.

Article Snippet: Recombinant FNDC5 protein (Cat No: H00252995-P01) was from Abnova.

Techniques: Expressing, Western Blot

Journal: Scientific Reports

Article Title: Gene Location, Expression, and Function of FNDC5 in Meishan Pigs

doi: 10.1038/s41598-017-08406-y

Figure Lengend Snippet: In vitro stimulation of proliferation of primary adipocytes by FNDC5 in Meishan pigs. ( A ) Lentiviral vector transfection efficiency. Red fluorescence represents cells stably transfected with FNDC5 interfering RNA. ( B ) FNDC5 expression levels as detected by qPCR in the control (NC) group and FNDC5 RNAi (Si) group in primary adipocytes of Meishan pigs. Data are reported as mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. ( C ) Western blot results of FNDC5 in control (NC) group and RNAi (Si) group of primary adipocytes from Meishan pigs. ( D ) CCK8 assay was used to measure the proliferation of primary adipocytes in control (red line) group and RNAi (green line) group. Data are reported as mean ± SEM (n = 4), *P < 0.05; **P < 0.01. ( E ) CCK8 assay was used to measure the proliferation of primary adipocytes in the control (red line) group and FNDC5-treared (blue line) group. Data are reported as mean ± SEM (n = 4). *P < 0.05, **P < 0.01.

Article Snippet: Recombinant FNDC5 protein (Cat No: H00252995-P01) was from Abnova.

Techniques: In Vitro, Plasmid Preparation, Transfection, Fluorescence, Stable Transfection, Expressing, Control, Western Blot, CCK-8 Assay

Journal: Scientific Reports

Article Title: Gene Location, Expression, and Function of FNDC5 in Meishan Pigs

doi: 10.1038/s41598-017-08406-y

Figure Lengend Snippet: In vitro stimulation of adipogenic differentiation by FNDC5 in porcine primary adipocytes. ( A ) Photos showing Meishan porcine adipocytes following adipogenic differentiation on day 8. ( B ) Photos showing Meishan porcine adipocytes stained with oil red O following adipogenic differentiation on day 8. ( C ) The qPCR result of PPARγ. Data are reported as mean ± SEM (n = 3). *P < 0.05, **P < 0.01. Si: RNAi transfected group; NC: control plasmid transfected group; FNDC5: control plasmid transfected group supplemented with recombinant FNDC5 protein.

Article Snippet: Recombinant FNDC5 protein (Cat No: H00252995-P01) was from Abnova.

Techniques: In Vitro, Staining, Transfection, Control, Plasmid Preparation, Recombinant

Journal: Scientific Reports

Article Title: Gene Location, Expression, and Function of FNDC5 in Meishan Pigs

doi: 10.1038/s41598-017-08406-y

Figure Lengend Snippet: Enhancement of the browning of primary adipocytes by FNDC5 in Meishan pigs. Panels (A,B and D) represent the expression levels of marker genes for white fat, brown fat, and beige fat, respectively. Red column: RNAi transfected group; green column: control plasmid transfected group; blue column: control plasmid transfected group supplemented with recombinant FNDC5 protein. Data are reported as mean ± SEM (n = 3). *P < 0.05, **P < 0.01. ( C ) Western blot of browning marker gene products. Si: RNAi transfected group; NC: control plasmid transfected group; FNDC5: control plasmid transfected group supplemented with recombinant FNDC5 protein.

Article Snippet: Recombinant FNDC5 protein (Cat No: H00252995-P01) was from Abnova.

Techniques: Expressing, Marker, Transfection, Control, Plasmid Preparation, Recombinant, Western Blot

Journal: Scientific Reports

Article Title: Gene Location, Expression, and Function of FNDC5 in Meishan Pigs

doi: 10.1038/s41598-017-08406-y

Figure Lengend Snippet: Primer sequences used for RT-PCR and qPCR.

Article Snippet: Recombinant FNDC5 protein (Cat No: H00252995-P01) was from Abnova.

Techniques: Sequencing